Identification of Genetic Variants in Y-Chromosome Specific Genes Associated with Seminal Production in Murrah Buffaloes

Background: Marker assisted selection based strategies are essential to identify superior quality bulls at an early age for frozen semen production. The present study aims determine the genetic variants in Y-chromosome specific genes, sex determining region on (SRY) and testis protein Y-encoded (TSPY) association with production traits Murrah buffalo bulls. Methods: genomic DNA extracted from blood samples of 70 maintained under station were utilized PCR amplification. sequences SRY TSPY genes screened single nucleotide polymorphism using seqman module DNASTAR LASERGENE software. variations comparison reference phylogenetic tree construct obtained Clustal W MegAlign module. Result: analysis revealed that monomorphic nature, devoid specifying highly conserved among studied. Hence, studies seminal could not be performed. However, exon sequence gene showed a variation position 209 (G less than A) compared Nili-Ravi Kundi buffaloes. Similarly, exhibited significant number Bos taurus sequences. evinced close relationship divergence between other breeds species bovinae family. Further extensive SRY, large, diverse population help discover considerable SNP markers related breeding